YaxinBio a Supplier of Recombinant Carboxypeptidase B expressed in
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids
lysine, arginine and histidine from the C-terminal end of
polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the
optimum pH is 7.5-9.0. Carboxypeptidase B is competitively
inhibited by arginine and lysine. The enzyme is also inhibited by
metal chelating agents, e.g., EDTA. YaxinBio has cloned rat
carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal
original carboxypeptidase B .Recombinant carboxypeptidase B can
replace native carboxypeptidase B for using in a variety of
Single main band on SDS-PAGE
Less than 10 ppm of recombinant trypsin.
One Unit of carboxypeptidase B activity hydrolyzes one micromole of
hippuryl-L-arginine per minute at 25℃, pH 7.65.
Carboxypeptidase B (EC 22.214.171.124, protaminase, pancreatic
carboxypeptidase B, tissue carboxypeptidase B, peptidyl-L-lysine
[L-arginine]hydrolase) is a carboxypeptidase that preferentially
acts upon basic amino acids, such as arginine and lysine. This
serum enzyme is also responsible for rapidly metabolizing the C5a
protein into C5a des-Arg, with one less amino acid.
To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM
Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000
(w/w), the optimum pH is pH 7-9.
Recommend recombinant carboxypeptidase B lyophilized should be
stored under 2℃-8℃ in sealed container. It is stable within 24
months.After dissolved, it should be stored under -20℃, It is
stable within 24 months and above 90% activity remained after 10
times repeated freezing and thawing.
Complement and Coagulation Cascades, organism-specific biosystem;
Complement and coagulation cascades, conserved biosystem; Protein
digestion and absorption, organism-specific biosystem
carboxypeptidase activity; metallocarboxypeptidase activity; zinc